產(chǎn)品詳情
簡(jiǎn)單介紹：
重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白與其它公司提供的重組蛋白不同，rRtSDF-1β/CXCL12β蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白
| Species | Rat |
| Accession | Q9QZD1 |
| GeneID | 24772 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | 重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白Less than 1 EU/μg of rRtSDF-1β/CXCL12β as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠基質(zhì)細(xì)胞衍生因子-1 beta / CXCL12 beta蛋白 | |
| Reference |
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500. 2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9. 3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51. 4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9. 5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23. 6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703. 7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53. |
| Background | CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. In all SDF-1 isoforms, SDF-1β is the canonical sequence. It has the complete amino acids in the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, murine CXCL12β shares approximately 96 % amino acid sequence identity with human CXCL12β. |
